Scientific Publications

1. DE Wolf: Membrane behavior via fluorescence. The Cornell Engineer 42: 19-21, 1976.

2. PG Fahey, DE Koppel, LS Barak, DE Wolf, EL Elson and WW Webb: Lateral diffusion in planar lipid bilayers. Science 195: 305-306, 1976.

3. DE Wolf, J Schlessinger, EL Elson, WW Webb and P Henkart: Diffusion and patching of macromolecules on planar lipid bilayer membranes. Biochemistry 16: 3476-3483, 1977.

4. DE Wolf, M Edidin and PR Dragsten: The effect of bleaching light on measurements of lateral diffusion in cell membranes by the fluorescence photobleaching recovery method. PNAS USA 77: 2043-2045, 1980.

5. DE Wolf, P Henkart and WW Webb: The diffusion, patching, and capping of stearoyl dextrans on 3T3 cell plasma membranes. Biochemistry 19: 3893-3904, 1980.

6. RD Klausner and DE Wolf: Selectivity of fluorescent lipid analogues for lipid domains. Biochemistry 19: 6199-6203, 1980.

7. DE Wolf, W Kinsey, WJ Lennarz and M Edidin: Changes in the organization of the sea urchin egg plasma membrane upon fertilization: indications from the lateral diffusion of lipid soluble fluorescent dyes. Dev Biol 81: 133-138, 1981.

8. DE Wolf, M Edidin and AH Handyside: Changes in the organization of the mouse egg plasma membrane upon fertilization and first cleavage: indications from the lateral diffusion rates of fluorescence lipid analogues. Dev Biol 85: 195-198, 1981.

9. DE Wolf and M Edidin: Methods of measuring diffusion and mobility of molecules in surface membranes. In Techniques in Cellular Physiology, P. Baker, (Ed), Elsevier/North Holland Biomedical Press. Part 1, Volume P1/1 Part 105: 1-14, 1981.

10. DE Wolf, AH Handyside and M Edidin: Effect of surface microvilli on lateral diffusion measurements made by the fluorescence photobleaching recovery technique. Biophys J 38: 295-297, 1982.

11. DE Wolf: The plasma membrane in early mammalian development. Invited review in Development in Mammals. M.H. Johnsonn (Ed), Elsevier/North Holland Biomedical Press, Amsterdam. 5: 187-208, 1983.

12. MF Ethier, DE Wolf and D Melchior: A calorimetric investigation of the phase partitioning of fluorescent carbocyanine probes in phosphatidylcholine bilayers. Biochemistry 22: 1178-1182, 1983.

13. DE Wolf and CA Ziomek: Regionalization and lateral diffusion of membrane proteins in unfertilized and fertilized mouse eggs. JCell Biol 96: 1786-1790, 1983.

14. DE Wolf and JK Voglmayr: Diffusion and regionalization in membranes of maturing ram spermatozoa. J Cell Biol 98: 1678-1684, 1984.

15. DE Wolf: Determination of the sidedness of carbocyanine dye labelling of membranes. Biochemistry 24: 582-586, 1985.

16. BS Packard and DE Wolf: Fluorescence lifetimes of carbocyanine lipid analogues in phospholipid bilayers. Biochemistry 24: 5176-5181, 1985.

17. DE Wolf, SS Hagopian and S Ishijima: Changes in sperm plasma membrane lipid diffusibility following hyperactivation during in vitro capacitation in the mouse. J Cell Biol 102: 1372-1377, 1985.

18. DE Wolf, SS Hagopian, RG Lewis, JK Voglmayr and G Fairbanks: Lateral regionalization and diffusion of a maturation-dependent antigen in the ram sperm plasma. J Cell Biol 102: 1826-1831, 1985.

19. DE Wolf, BK Scott and CF Millette: The development of regionalized lipid diffusibility in the germ cell plasma membrane during spermatogenesis in the mouse. J Cell Biol 103: 1745-1750, 1986.

20. DE Wolf: Overcoming random diffusion in polarized cells - corralling the drunken beggar. Invited review, Bio Essays 6: 116-124, 1987.

21. S Triestman, M Moynihan and DE Wolf: Influence of alcohols, temperature and region on the mobility of lipids in neuronal membrane. Biochem Biophys Acta 898: 109-120, 1987.

22. AH Handyside, M Edidin and DE Wolf: Polarized distribution of membrane components on two-cell mouse embryos. Wilhelm Roux Archives of Developmental Biology 196: 273-278, 1987.

23. DE Wolf: Diffusion and the control of membrane regionalization in mammalian spermatozoa. Ann NY Acad Sci 513: 247-261, 1987.

24. DE Wolf: Probing the lateral organization and dynamics of membranes. Invited review in Spectroscopic Membrane Probes. L. Loew (Ed), CRC Press, Boca Raton, Fl, Vol I, pp. 193-220, 1988.

25. DE Wolf, AC Lipscomb and DE Maynard: Causes of non-diffusing lipid in the plasma membranes of mammalian spermatozoa. Biochemistry 27: 860-865, 1988.

26. RA Cardullo, S Agrawal, C Flores, P Zamecnik and DE Wolf: Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. PNAS USA 85: 8790-8794, 1988.

27. DE Wolf: Designing, building and using a fluorescence recovery after photobleaching instrument. Invited chapter in Quantitative Fluorescence Microscopy: Imaging and Spectroscopy. D.L. Taylor and Y.-l. Wang (Eds), Academic Press, NY, pp 271-306, 1989.

28. RA Cardullo and DE Wolf: The sperm plasma membrane: a little more than mosaic, a little less than fluid. Invited chapter in Structure and Function of Ciliary and Flagellar Surfaces. R.A. Bloodgood (Ed), Plenum Press, NY, pp 305-336, 1990.

29. DE Wolf, RA Cardullo and RM Mungovan: Using quantitative video microscopy to study mammalian sperm. In Video Microscopy. K. Jacobson and B. Herman (Eds), Wiley-Liss New York, NY, pp 373-382, 1990.

30. G Venkatakrishnan, CA McKinnon, AH Ross, and DE Wolf: Lateral diffusion of nerve growth factor receptor: modulation by ligand binding and cell associated factors. Cell Regulation 1: 605-614, 1990.

31. RA Cardullo, S Agrawal, KM Bocian, CA McKinnon and DE Wolf: Synthesis, purification and characterization of 2,4,6 trinitrophenyl UDP galactose: A fluorescent analogue of UDP galactose for studying galactosyl transferase localization and function. Anal Biochem 188: 305-309, 1990.

32. DE Wolf, VM Maynard, CA McKinnon and DL Melchior: Lipid domains in the ram sperm plasma membrane demonstrated by differential scanning calorimetry. PNAS USA 87: 6893-6896, 1990.

33. RA Cardullo, RM Mungovan and DE Wolf: Imaging membrane organization and dynamics. In Biophysical and Biochemical Aspects of Fluorescence Spectroscopy, G. Dewey (Ed), Plenum Press, NY, pp 231-260, 1991.

34. CA McKinnon, FE Weaver, JA Yoder, G Fairbanks and DE Wolf: Cross-linking a maturation-dependent ram sperm plasma membrane antigen induces the acrosome reaction. Mol Reprod Dev 29: 200-207, 1991.

35. G Venkatakrishnan, CA McKinnon, CG Pilapil, DE Wolf and AH Ross: Nerve growth factor receptors are preaggregated and immobile on responsive cells. Biochemistry 30: 2748-2753, 1991.

36. RA Cardullo and DE Wolf: Biophysical determinants of cell surface events leading to sperm-zona adhesion and induction of the acrosome reaction. Serono Symposia Publications, Vol. 75, B. Baccotti (Ed), Raven Press, NY, 1991.

37. DE Wolf and RA Cardullo: Physical Properties of the mammalian sperm plasma membrane. Serono Symposia Publications, Vol. 75, B. Baccoti (Ed), Raven Press, NY, 1991.

38. DE Wolf: Theory of fluorescence recovery after photobleaching measurements on cylindrical surfaces. Biophys J 61: 1-7, 1992.

39. DE Wolf, A Winiski, A Ting, KM Bocian and RE Pagano: Determination of the trans bilayer distribution of fluorescent lipid analogues by nonradiative fluorescence resonance energy transfer. Biochemistry, 31: 2865-2873, 1992.

40. DE Wolf, CA McKinnon, K Lakoski Loveland, L Leyton and PM Saling: Protein dynamics in sperm membranes: implications for sperm function during gamete interaction. Mol Reprod Dev 33: 228-234, 1992.

41. DE Wolf: Lipid domains - the parable of the blind man and the elephant. Comm Molec Cell Biophys 8: 83-95, 1992.

42. FE Weaver, JE Dino, BJ Germain, DE Wolf and G Fairbanks: Biochemical characterization and epididymal localization of the maturation-dependent ram sperm surface antigen ESA152. Mol Reprod Dev 35: 293-301, 1993.

43. DE Wolf: Microheterogeneity in biological membranes. In: Current Topics in Membranes, Vol 40, D. Hoekstra (Ed), Academic Press, Orlando, FL pp.143-165, 1994.

44. G Sluder, F Miller, CA Thompson and DE Wolf: Feedback control of metaphase-anaphase transition in sea urchins zygotes: role of maloriented chromosomes. J Cell Biol 126: 189-198, 1994.

45. DE Wolf: Lipid Domains in Sperm Plasma Membranes. Mol Memb Biol 12: 101-104, 1995.

46. DE Wolf, CA McKinnon, MC Daou, RM Stephens, DR Kaplan and AH Ross: Interaction with TrkA immobilizes gp75 in the high affinity nerve growth factor receptor complex. J Biol Chem 270: 2133-2138, 1995.

47. DE Wolf, CA McKinnon, MC Daou, RM Stephens, DR Kaplan and AH Ross: The neurotrophin receptor, gp75, forms a complex with the receptor tyrosine kinase TrkA. J Cell Biol 132: 945-953, 1995.

48. RA Cardullo and DE Wolf: Distribution and dynamics of mouse sperm surface galactosyltransferase: implications for mammalian fertilization. Biochemistry, 34: 10027-10035, 1995.

49. AH Ross, CA McKinnon, MC Daou, K Ratliff and DE Wolf: Differential biological effects of K252 kinase inhibitors are related to membrane solubility but not to permeability. J Neuro Chem 65: 2748-2756, 1995.

50. AH Ross, MC Daou, CA McKinnon, PJ Condon, MB Lachyankar, RM Stephens, DR Kaplan and DE Wolf: A complex of gp75 and TrKA Nerve Growth Factor Receptors demonstrated by copatching. J Cell Biol 132: 945-954, 1995.

51. TA Perkins, DE Wolf and J Goodchild: Fluorescence resonance energy transfer analysis of ribozyme kinetics reveals the mode of action of a facilitator oligonucleotide. Biochemistry, 35: 16370-16377, 1996.

52. DE Wolf, CA McKinnon-Thompson, MC Daou, RM Stephens and AH Ross: Mobility of TrkA is regulated by phosphorylation and interactions with the low-affinity NGF receptor. Biochemistry, 37:3178-86, 1998.

53. TT Smith, CA McKinnon-Thompson and DE Wolf: Changes in lipid diffusibility in the hamster sperm head plasma membrane during capacitation in vitro and in vivo. Mol Reprod Dev, 50: 86-92, 1998.

54. DE Wolf: Quantitative Video Microscopy. In Methods in Cell Biology, Video Microscopy, G Sluder and DE Wolf (Eds), Academic Press, N Y 56: 117-134, 1998.

55. G Sluder and DE Wolf: (Eds), Methods in Cell Biology, Video Microscopy. Academic Press, NY, 1998.

56. JC Politz, ES Browne, DE Wolf and T Pederson: Intranuclear diffusion and hybridization state of oligonucleotides measured by fluorescence correlation spectroscopy in living cells. PNAS (USA) 95: 6043-6048, 1998.

57. DE Wolf: Diabetes: Novel Therapeutic Developments. Invited to review meeting 1999, London, UK. IDrugs 2: 1292-1294, 1999.

58. G Sluder and DE Wolf: (Eds), Methods in Cell Biology, Digital Microscopy. Elsevier/Academic Press, NY, 2003.

59. DE Wolf: Quantitative Digital and Video Microscopy. In Methods in Cell Biology, Digital Microscopy, G Sluder and DE Wolf (Eds), Elsevier/Academic Press, N Y 56: 319-336, 2003.

60. DA Bulseco and DE Wolf: Fluorescence Correlation Spectroscopy: Molecular Complexing in Solution and in Living Cells. In Methods in Cell Biology, Digital Microscopy, G Sluder and DE Wolf (Eds), Elsevier/Academic Press, N Y 56: 465-498, 2003.

61. DE Wolf: Fundamentals of Fluorescence and Fluorescence Microscopy. In Methods in Cell Biology, Digital Microscopy, G Sluder and DE Wolf (Eds), Elsevier/Academic Press, N Y 56: 157-184, 2003.

62. DE Wolf: The Optics of Microscope Image Formation. In Methods in Cell Biology, Digital Microscopy, G Sluder and DE Wolf (Eds), Elsevier/Academic Press, N Y 56: 11-43, 2003.

Scientific Abstracts

A1. DE Wolf, J Schlessinger, EL Elson, WW Webb, R Blumenthal and P Henkart: Diffusion and patching of macromolecules on planar lipid bilayer membranes. 21st Meeting Biophysical Soc. New Orleans, LA, 1977.

A2. DE Wolf, P Henkart and WW Webb: Diffusion, patching and capping of synthetic antigens on model and 3T3 cell plasma membranes. 22nd Meeting Biophysical Soc. Washington, DC, 1978.

A3. DE Wolf, M Edidin, W Kinsey and WJ Lennarz: Changes in the diffusion of protein and lipid probes on the sea urchin egg plasma membrane. 19th Meeting ASCB, Toronto, 1979.

A4. DE Wolf and RD Klausner: Selectivity for fluorescent lipid analogues for lipid domains. 24th Meeting Biophysical Soc., New Orleans, LA, 1980.

A5. M Edidin and DE Wolf: Changes in the organization of egg plasma membranes upon fertilization: evidence for domains in lipid bilayers. Jost Symposium on Perspectives in Differentiation and Hypertrophy, Runaway Bay, Jamaica, WI, 1980.

A6. DE Wolf and RD Klausner: Selectivity of fluorescent lipid analogues for lipid domains. Invited speaker, minisymposium on Lateral Organization in Membranes. 25th Annual Meeting Biophysical Soc., Denver, CO, 1981.

A7. AH Handyside, DE Wolf and M Edidin: Polarization of membrane components following first cleavage of the mouse egg. British Society for Developmental Biololgy, UK, 1981.

A8. DE Wolf and R Klausner: Selectivity of fluorescent lipid analogues for lipid domains. Invited speaker at the International Workshop on the Biological Applications of Photobleaching Techniques. Chapel Hill, NC, 1981.

A9. DE Wolf and M Edidin: Changes in the organization of the embryo plasma membrane upon fertilization and early development: indications from the lateral diffusion rates of fluorescent lipid analogs. Invited speaker at the International Workshop on the Biological Applications of Photobleaching Techniques. Chapel Hill, NC, 1981.

A10. CA Ziomek and DE Wolf: Cell membrane regionalization and protein mobility in preimplantation mouse embryos. EMBO Workshop on Organizational Membrane Polarity in Epithelia. Arolla, Switzerland, 1982.

A11. DE Wolf: Probing the domain organization of membrane lipids. Invited speaker at workshop on Applications of Photobleaching Techniques to Cell Biology. 22nd Meeting ASCB. Baltimore, MD, 1982.

A12. DE Wolf, SS Hagopian and J Voglmayr: Diffusion and regionalization in membranes of maturing ram sperm. 22nd Meeting ASCB. Baltimore, MD, 1982.

A13. CA Ziomek and DE Wolf: Membrane regionalization and protein mobility in preimplantation mouse embryos. 22nd Meeting ASCB. Baltimore, MD, 1982.

A14. MF Ethier, DE Wolf and DL Melchior: A calorimetric investigation of the phase partitioning of the fluorescent carbocyanine probes in phosphatidylcholine bilayers. 27th Meeting Biophysical Society. San Diego, CA, 1983.

A15. BS Packar and DE Wolf: Fluorescence lifetimes and fluorescence yields of carbocyanine probes in phospholipid bilayers. 28th Biophysical Society Meeting. San Antonio, TX, 1984.

A16. DA Williams, DE Wolf and FS Fay: Determination of ionized calcium concentration in smooth muscle cells using Quin 2. American Physiology Society Meeting. Woods Hole, MA, 1984.

A17. CA Ziomek, M Lepire, M Moynihan and DE Wolf: Membrane protein and lipid regionalization in 8-Cell-mouse embryos. 24th Cell Biology Meeting. Kansas City, 1984.

A18. DE Wolf and SS Hagopian: Changes in lipid diffusion with capacitation in mouse sperm. 29th Biophysical Society Meeting. Baltimore, MD, 1985.

A19. DE Wolf, SS Hagopian, RG Lewis, J Voglmayr and G Fairbanks: Diffusion and regionalization of a surface glycolipid of ejaculated ram spermatozoa. 29th Biophysical Society Meeting. Baltimore, MD, 1985.

A20. DE Wolf: Diffusion and control of membrane organization, State of the Art Lecture Membrane Fluidity - a Biophysical and Cytochemical Appraisal, 99th American Association of Anatomists Meeting. Reno, NV, 1986.

A21. CA Ziomek, M Lepire, M Moynihan and DE Wolf: Preimplantion mouse embryos express a human placental like alkaline phosphatase. 26th ASCB Meeting. Washington, DC, 1986.

A22. S Agrawal, RA Cardullo, PC Zamecnik and DE Wolf: Probing nucleic acid structure by non-radiative fluorescence resonance energy transfer. 28th ASCB Meeting. San Francisco, CA, 1989.

A23. DE Wolf, RA Cardullo, CA McKinnon, KA Lakoski and PM Saling: Diffusion of proteins in the head plasma membrane of mouse spermatozoa. 28th ASCB Meeting, San Francisco, CA, 1989.

A24. RA Cardullo, S Agrawal CA McKinnon and DE Wolf: Detection of mouse sperm galactosyl transferase using a fluorescent analogue of UDP-galactose. 28th ASCB Meeting. San Francisco, CA, 1989.

A25. G Venkatakrishnan, CA McKinnon, DE Wolf and AH Ross: Lateral diffusion of NGF receptors expressed on human melanoma and COS cells. 29th ASCB Meeting. Houston, TX, 1989.

A26. AH Ross, G Venkatakrishnan, CA McKinnon and DE Wolf: Correlation of nerve growth factor receptor distribution and diffusibility with responsiveness and ligand-binding affinity. 20th Annual Meeting of Society for Neuroscience. St. Louis, MO, 1990.

A27. RA Cardullo, S Agrawal, J Yoder and DE Wolf: Structure of duplex oligodeoxynucleotides using non-radiative fluorescence resonance energy transfer. 35th Annual Meeting of Biophysical Society. San Francisco, CA, 1991.

A28. RA Cardullo, CA McKinnon and DE Wolf: Distribution and mobility of mouse sperm surface b-1, 4-galactosyltransferase using monovalent fluorescent probes. 35th Annual Meeting of Biophysical Society. San Francisco, CA, 1991.

A29. DE Wolf, CA McKinnon, R Stephens and AH Ross: Interaction of gp75 and gp140trk in the high affinity nerve growth factor receptor. 37th Annual Meeting of Biophysical Society, Washington, DC, 1993.

A30. AH Ross, MC Daou, DR Kaplan, MB Lachyankar, CA McKinnon, DK Poluha, W Poluha, R Stephens and DE Wolf: TrkA interacts with high-affinity NGF receptor and regulates neurodifferentiation. 23rd Annual Meeting Society for Neuroscience. Washington, DC, 1993.

A31. DE Wolf, CA McKinnon, MC Daou, D Kaplan, R Stephens, M Condon, M Lachyankar and AH Ross: Interaction of gp75 and gp140Trk on the high-affinity nerve growth factor receptor. 3rd International Meeting on NGF. Banff, Canada, 1994.

A32. AH Ross, DK Poluha, W Poluha, MCDoau, CA McKinnon, K Ratliff and DE Wolf: K252 compounds, TrkA and Neuronal differentiation. 24th Annual Meeting Society for Neurosciences. Miami Beach, FL, 1994.

A33. DE Wolf, CA McKinnon, MC Daou, DR Kaplan, RM Stephens, PJ Condon, MB Lachyankar and AH Ross: Interaction of gp75 and gp140Trk in the high-affinity nerve growth factor receptor. 34th Annual Meeting American Society for Cell Biology. San Francisco, CA, 1994.

A34. CA McKinnon, FE Weaver, ES Browne, O Braha and DE Wolf: Characterization of a maturation dependent ram sperm antigen and reconstitution of its activity into heterologous sperm. 34th Annual Meeting American Society for Cell Biology. San Francisco, CA, 1994.

A35. DE Wolf, CA McKinnon, MC Daou, PJ Condon, MB Lachyankar, AH Ross, DR Kaplan and RM Stephens: Nerve growth factor receptor dynamics and subunit association studied by fluorescence recovery after photobleaching, copatching, and site directed mutagenesis. 39th Annual Meeting of Biophysical Society. San Francisco, CA, 1995.

A36. DE Wolf, CA McKinnon, K Ratliff, MC Daou and AH Ross: Differential action of fluorescent tyr-kinase inhibitors K252A and K252B is related to membrane solubility not permeability. 39th Annual Meeting of Biophysical Society. San Francisco, CA, 1995.

A37. CA McKinnon, E Brown, F Weaver, O Braha and DE Wolf: Vesicle to plasma membrane transfer of a hydrophobic ram sperm protein ESA152 to heterologous sperm leads to activation of acrosomal exocytosis upon antibody crosslinking. 39th Annual Meeting of Biophysical Society. San Francisco, CA, 1995.

A38. AH Ross, CA McKinnon, MC Daou, RM Stephens, DR Kaplan and DE Wolf: Identification of a nerve growth factor receptor complex by biophysical approaches. Annual Mtg. Society for Neuroscience. San Diego, CA, 1995.

A39. CA McKinnon, FE Weaver, ES Browne, O Braha and DE Wolf: Characterization of a maturation dependent ram sperm antigen and reconstitution of its activity into heterologous sperm. Gordon Research Conference on Fertilization and Activation of Development. 1995.

A40. TA Perkins, J Goodchild, DE Wolf: Kinetics of hammerhead ribozyme/substrate association and dissociation in the presence of oligonucleotide facilitators using fluorescence resonance energy transfer. Meeting on RNA processing, Cold Spring Harbor Laboratory. Cold Spring Harbor, NY, 1995.

A41. DE Wolf, CA McKinnon, MC Daou, DR Kaplan, RM Stephens, PJ Condon, MB Lachyankar and AH Ross: Interaction of gp75 and gp140Trk in the high-affinity nerve growth factor receptor. Gordon Research Conference on Neurotrophins, 1995.

A42. DE Wolf: Optical fluorescence subcutaneous glucose sensor. Association for the Advancement of Medical Instrumentation, Artificial Pancreas Symposium, 1999 Annual Meeting. Boston, MA, 1999.

A43. DE Wolf: Long-term care through islet cell transplant therapy. SMi Conference: Diabetes: Novel Therapeutic Developments. London, England, 1999.

A44. DE Wolf: Treatment of diabetes with immunoisolating microreactors. IBC’s 5th Annual International Conference on Xenotransplantation. Boston, MA, 1999.

A45. DE Wolf: Workshop on fluorescence correlation spectroscopy. The American Society for Cell Biology, 39th Annual Meeting. Washington, DC, 1999.

A46. D Bulseco, CA Thompson, ES Browne, PR Quinby, DE Wolf: Global analysis of FCS data: diffusion of DilC16 and gp75 in the plasma membrane of A875 human melanoma cells. Biophysical Society, 44th Annual Meeting. New Orleans, LA, 2000.